2022
A mechanism of origin licensing control through autoinhibition of S. cerevisiae ORC·DNA·Cdc6
Schmidt JM, Yang R, Kumar A, Hunker O, Seebacher J, Bleichert F. A mechanism of origin licensing control through autoinhibition of S. cerevisiae ORC·DNA·Cdc6. Nature Communications 2022, 13: 1059. PMID: 35217664, PMCID: PMC8881611, DOI: 10.1038/s41467-022-28695-w.Peer-Reviewed Original ResearchConceptsOrigin recognition complexS. cerevisiaeCyclin-dependent kinase phosphorylationMcm2-7 loadingN-terminal domainCryo-electron microscopyCDK phosphorylationRecognition complexDNA replicationReplication originsÅ resolutionKinase phosphorylationMechanism of originCdc6Coordinated actionCerevisiaePhosphorylationDNAInhibitory signalsStructural detailsSite regulationRecruitmentOrc6AssemblyCdt1
2013
A Meier-Gorlin syndrome mutation in a conserved C-terminal helix of Orc6 impedes origin recognition complex formation
Bleichert F, Balasov M, Chesnokov I, Nogales E, Botchan MR, Berger JM. A Meier-Gorlin syndrome mutation in a conserved C-terminal helix of Orc6 impedes origin recognition complex formation. ELife 2013, 2: e00882. PMID: 24137536, PMCID: PMC3791464, DOI: 10.7554/elife.00882.Peer-Reviewed Original ResearchConceptsOrigin recognition complexMetazoan origin recognition complexMeier-Gorlin syndrome mutationsORC functionMcm2-7 loadingC-terminal helixMeier-Gorlin syndromeReplisome formationUncharacterized domainYeast complexRecognition complexDNA replicationEnigmatic functionSubunit organizationProper recruitmentBioinformatics analysisChromosomal originC-terminusOrc6Biochemical studiesCertain subunitsMutationsComplex formationSubunitsGlobal architecture