2021
Hyperosmotic stress alters the RNA polymerase II interactome and induces readthrough transcription despite widespread transcriptional repression
Rosa-Mercado NA, Zimmer JT, Apostolidi M, Rinehart J, Simon MD, Steitz JA. Hyperosmotic stress alters the RNA polymerase II interactome and induces readthrough transcription despite widespread transcriptional repression. Molecular Cell 2021, 81: 502-513.e4. PMID: 33400923, PMCID: PMC7867636, DOI: 10.1016/j.molcel.2020.12.002.Peer-Reviewed Original ResearchConceptsWidespread transcriptional repressionTranscriptional repressionPol IIIntegrator complex subunitsRNA polymerase IIGenome-wide lossStress-induced redistributionParental genesTranscriptional outputDoG inductionPolymerase IIChIP sequencingHuman cell linesUpstream geneComplex subunitsPolyadenylation factorsTranscription profilesReadthrough transcriptsCatalytic subunitIntegrator activityCellular stressHyperosmotic stressTranscriptional levelTranscription resultsGenes
1993
A general two-metal-ion mechanism for catalytic RNA.
Steitz TA, Steitz JA. A general two-metal-ion mechanism for catalytic RNA. Proceedings Of The National Academy Of Sciences Of The United States Of America 1993, 90: 6498-6502. PMID: 8341661, PMCID: PMC46959, DOI: 10.1073/pnas.90.14.6498.Peer-Reviewed Original ResearchConceptsMetal ionsTwo-metal-ion mechanismCatalytic metal ionPhosphoryl transfer reactionsChemical catalysisLewis acidTransfer reactionsReaction pathwaysTransition stateProtein enzymesCatalytic siteSugar hydroxylsIonsGroup I self-splicing intronCatalytic RNAReactionSelf-splicing intronsP hydrolysisCatalysisBinding sitesOxyanionsHydroxylSpecific binding sitesHydrolysisAcid
1991
Structural analyses of the 7SK ribonucleoprotein (RNP), the most abundant human small RNP of unknown function.
Wassarman DA, Steitz JA. Structural analyses of the 7SK ribonucleoprotein (RNP), the most abundant human small RNP of unknown function. Molecular And Cellular Biology 1991, 11: 3432-3445. PMID: 1646389, PMCID: PMC361072, DOI: 10.1128/mcb.11.7.3432.Peer-Reviewed Original Research
1990
Spliced leader RNA sequences can substitute for the essential 5′ end of U1 RNA during splicing in a mammalian in vitro system
Bruzik J, Steitz J. Spliced leader RNA sequences can substitute for the essential 5′ end of U1 RNA during splicing in a mammalian in vitro system. Cell 1990, 62: 889-899. PMID: 2168293, DOI: 10.1016/0092-8674(90)90264-f.Peer-Reviewed Original ResearchThe U3 small nucleolar ribonucleoprotein functions in the first step of preribosomal RNA processing
Kass S, Tyc K, Steitz J, Sollner-Webb B. The U3 small nucleolar ribonucleoprotein functions in the first step of preribosomal RNA processing. Cell 1990, 60: 897-908. PMID: 2156625, DOI: 10.1016/0092-8674(90)90338-f.Peer-Reviewed Original ResearchAnimalsBase SequenceBlotting, NorthernCarcinoma, Ehrlich TumorCell NucleolusDNA, RibosomalEndoribonucleasesMiceMolecular Sequence DataNucleic Acid ConformationOligonucleotide ProbesRibonuclease HRibonucleoproteinsRibonucleoproteins, Small NuclearRNA PrecursorsRNA Processing, Post-TranscriptionalRNA, Small NuclearTemplates, Genetic
1989
The mammalian analogue of the yeast PRP8 splicing protein is present in the U4/5/6 small nuclear ribonucleoprotein particle and the spliceosome.
Pinto AL, Steitz JA. The mammalian analogue of the yeast PRP8 splicing protein is present in the U4/5/6 small nuclear ribonucleoprotein particle and the spliceosome. Proceedings Of The National Academy Of Sciences Of The United States Of America 1989, 86: 8742-8746. PMID: 2479028, PMCID: PMC298364, DOI: 10.1073/pnas.86.22.8742.Peer-Reviewed Original ResearchConceptsSmall nuclear ribonucleoproteinU5 small nuclear ribonucleoproteinHeLa cell nuclear extractsSmall nuclear ribonucleoprotein particleCell nuclear extractsAnti-trimethylguanosine antibodyNuclear ribonucleoprotein particleAffinity-purified spliceosomesSplicing proteinsMammalian proteinsYeast proteinsSnRNP complexMRNA splicingSplicing extractsRibonucleoprotein particleNuclear ribonucleoproteinMammalian analogueNuclear extractsSm classGradient fractionationSm epitopesProteinSpliceosomeProtein reactivePrp8[31] Determination of RNA-protein and RNA-ribonucleoprotein interactions by nuclease probing
Parker K, Steitz J. [31] Determination of RNA-protein and RNA-ribonucleoprotein interactions by nuclease probing. Methods In Enzymology 1989, 180: 454-468. PMID: 2482428, DOI: 10.1016/0076-6879(89)80117-x.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAntibodiesCarrier ProteinsElectrophoresis, Polyacrylamide GelEndoribonucleasesHumansIndicators and ReagentsKineticsMiceMicrococcal NucleaseNucleotide MappingRibonuclease HRibonuclease T1RibonucleasesRibonucleoproteinsRNARNA PrecursorsRNA SplicingRNA-Binding ProteinsRNA, Ribosomal, 28STranscription, Genetic
1988
An in vitro interaction between the human U3 snRNP and 28S rRNA sequences near the α-sarcin site
Parker K, Bruzik J, Steitz J. An in vitro interaction between the human U3 snRNP and 28S rRNA sequences near the α-sarcin site. Nucleic Acids Research 1988, 16: 10493-10509. PMID: 2974535, PMCID: PMC338920, DOI: 10.1093/nar/16.22.10493.Peer-Reviewed Original ResearchAntibodiesBase CompositionBase SequenceEndoribonucleasesFungal ProteinsHeLa CellsHumansMolecular Sequence DataNucleic Acid ConformationPlasmidsProtein Synthesis InhibitorsRibonuclease T1RibonucleoproteinsRibonucleoproteins, Small NuclearRNA PrecursorsRNA, RibosomalRNA, Ribosomal, 28STranscription, Genetic
1987
Identification of the Human U7 snRNP as One of Several Factors Involved in the 3′ End Maturation of Histone Premessenger RNA's
Mowry K, Steitz J. Identification of the Human U7 snRNP as One of Several Factors Involved in the 3′ End Maturation of Histone Premessenger RNA's. Science 1987, 238: 1682-1687. PMID: 2825355, DOI: 10.1126/science.2825355.Peer-Reviewed Original ResearchConceptsU7 snRNPPre-mRNAEnd processingDownstream elementsCleavage siteSmall nuclear ribonucleoprotein complexesMammalian pre-mRNAHeLa cell extractsNuclear ribonucleoprotein complexesHistone pre-mRNAEnd maturationEukaryotic cellsRibonucleoprotein complexesPremessenger RNARNA moietySplicing reactionGene transcriptsCell extractsSnRNPMessenger RNARNA
1985
U2 as well as U1 small nuclear ribonucleoproteins are involved in premessenger RNA splicing
Black D, Chabot B, Steitz J. U2 as well as U1 small nuclear ribonucleoproteins are involved in premessenger RNA splicing. Cell 1985, 42: 737-750. PMID: 2996775, DOI: 10.1016/0092-8674(85)90270-3.Peer-Reviewed Original Research