2023
Hedgehog costimulation during ischemia-reperfusion injury potentiates cytokine and homing responses of CD4+ T cells
Wang S, Song G, Barkestani M, Tobiasova Z, Wang Q, Jiang Q, Lopez R, Adelekan-Kamara Y, Fan M, Pober J, Tellides G, Jane-wit D. Hedgehog costimulation during ischemia-reperfusion injury potentiates cytokine and homing responses of CD4+ T cells. Frontiers In Immunology 2023, 14: 1248027. PMID: 37915586, PMCID: PMC10616247, DOI: 10.3389/fimmu.2023.1248027.Peer-Reviewed Original ResearchConceptsIschemia-reperfusion injuryHuman skin xenograftsSkin xenograftsT cellsPolyfunctional cytokine responsesSolid organ transplantationT cell subsetsResponse of CD4Expression of ICOST cell populationsHumanized mouse modelPeripheral helper cellsAllograft lossIL-21PD-1Reperfusion injuryCytokine responsesVascular inflammationPolyclonal expansionHelper cellsOrgan transplantationMouse modelClinical problemCostimulatory signalsDistinct subsetsA ZFYVE21-Rubicon-RNF34 signaling complex promotes endosome-associated inflammasome activity in endothelial cells
Li X, Jiang Q, Song G, Barkestani M, Wang Q, Wang S, Fan M, Fang C, Jiang B, Johnson J, Geirsson A, Tellides G, Pober J, Jane-wit D. A ZFYVE21-Rubicon-RNF34 signaling complex promotes endosome-associated inflammasome activity in endothelial cells. Nature Communications 2023, 14: 3002. PMID: 37225719, PMCID: PMC10209169, DOI: 10.1038/s41467-023-38684-2.Peer-Reviewed Original ResearchConceptsEndothelial cellsInflammasome activityMembrane attack complexCaspase-1Potential therapeutic targetChronic rejectionComplement membrane attack complexTissue inflammationNLRP3 inflammasomeTissue injuryMouse modelTherapeutic targetDependent mannerInflammationAttack complexInflammasomeHuman tissuesFlightless IInhibitory associationsSkin modelRNF34CellsHedgehog-induced ZFYVE21 promotes chronic vascular inflammation by activating NLRP3 inflammasomes in T cells
Jiang B, Wang S, Song G, Jiang Q, Fan M, Fang C, Li X, Soh C, Manes T, Cheru N, Qin L, Ren P, Jortner B, Wang Q, Quaranta E, Yoo P, Geirsson A, Davis R, Tellides G, Pober J, Jane-Wit D. Hedgehog-induced ZFYVE21 promotes chronic vascular inflammation by activating NLRP3 inflammasomes in T cells. Science Signaling 2023, 16: eabo3406. PMID: 36943921, PMCID: PMC10061549, DOI: 10.1126/scisignal.abo3406.Peer-Reviewed Original ResearchConceptsIschemia-reperfusion injuryChronic vascular inflammationT cellsNLRP3 inflammasomeVascular inflammationChronic inflammationEndothelial cellsIFN-γ responsesControl T cellsNLRP3 inflammasome activityT memory cellsAllograft vasculopathyVascular sequelaeHuman endothelial cellsCoronary arteryEffector responsesCell-autonomous roleInflammasome activityMouse modelInflammationPatient samplesVigorous recruitmentInflammasomePrimary human cellsImmune signaling
2021
ZFYVE21 is a Mediator of Non-Canonical Hedgehog Signaling Activating NLRP3 Inflammasomes in a Pathologic Subset of CD4+PD-1hi T Cells
Jiang B, Fang C, Soh C, Li X, Geirsson A, Tellides G, Pober J, Jane-Wit D. ZFYVE21 is a Mediator of Non-Canonical Hedgehog Signaling Activating NLRP3 Inflammasomes in a Pathologic Subset of CD4+PD-1hi T Cells. The Journal Of Heart And Lung Transplantation 2021, 40: s136. DOI: 10.1016/j.healun.2021.01.422.Peer-Reviewed Original ResearchT cellsNLRP3 inflammasomeEndothelial cellsDelayed graft functionSubset of CD4Humanized mouse modelComplement-mediated injuryNLRP3 inflammasome activityHuman lymphoid cellsNon-canonical Hedgehog signallingNon-canonical hedgehogGraft functionPeripheral injuryMemory CD4Human endothelial cellsPD-1Injury showCellular immunityVascular injuryChemokine receptorsTissue injuryPrimary human endothelial cellsInflammasome activityMouse modelLymphoid cells
2019
ZFYVE21 is a Novel Complement-Induced Rab5 Effector Protein Mediating Cardiac Allograft Vasculopathy
Jane-Wit D, Fang C, Liu L, Qin L, Li G, Tellides G, Pober J. ZFYVE21 is a Novel Complement-Induced Rab5 Effector Protein Mediating Cardiac Allograft Vasculopathy. The Journal Of Heart And Lung Transplantation 2019, 38: s249. DOI: 10.1016/j.healun.2019.01.618.Peer-Reviewed Original ResearchCardiac allograft vasculopathyAllograft vasculopathyHumanized mouse modelMembrane attack complexNF-κB-inducing kinaseTransplant biopsiesMouse modelEC activationHuman coronary artery segmentsEndothelial cellsAntibody-mediated rejectionTransplant organ rejectionCoronary artery segmentsHuman lymphoid cellsVascular inflammationOrgan rejectionArtery segmentsPharmacologic alterationsVasculopathyLymphoid cellsNF-kBClinical relevance
2010
Graft-derived IL-6 Promotes Allogeneic T Cell Intimal Infiltration and Intimal Expansion in a Chimeric Mouse Model of Human Artery Rejection
Fogal B, Rao D, Yi T, Tellides G, Kulkarni S, Pober J. Graft-derived IL-6 Promotes Allogeneic T Cell Intimal Infiltration and Intimal Expansion in a Chimeric Mouse Model of Human Artery Rejection. Clinical Immunology 2010, 135: s29. DOI: 10.1016/j.clim.2010.03.091.Peer-Reviewed Original ResearchGraft-derived IL-6 Promotes Allogeneic T Cell Intimal Infiltration and Intimal Expansion in a Chimeric Mouse Model of Human Artery Rejection
Fogal B, Rao D, Yi T, Tellides G, Kulkarni S, Pober J. Graft-derived IL-6 Promotes Allogeneic T Cell Intimal Infiltration and Intimal Expansion in a Chimeric Mouse Model of Human Artery Rejection. Clinical Immunology 2010, 135: s42. DOI: 10.1016/j.clim.2010.03.127.Peer-Reviewed Original Research
2000
Selective Inhibition of NF-κB Activation by a Peptide That Blocks the Interaction of NEMO with the IκB Kinase Complex
May M, D'Acquisto F, Madge L, Glöckner J, Pober J, Ghosh S. Selective Inhibition of NF-κB Activation by a Peptide That Blocks the Interaction of NEMO with the IκB Kinase Complex. Science 2000, 289: 1550-1554. PMID: 10968790, DOI: 10.1126/science.289.5484.1550.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acid SequenceAnimalsAnti-Inflammatory Agents, Non-SteroidalCells, CulturedCOS CellsEndothelium, VascularE-SelectinGene Expression RegulationHeLa CellsHumansI-kappa B KinaseInflammationMiceMice, Inbred C57BLMolecular Sequence DataMutationNF-kappa BPeptidesPoint MutationProtein Serine-Threonine KinasesProtein Structure, TertiaryRecombinant Fusion ProteinsConceptsNF-kappaBBasal NF-kappaB activityExperimental mouse modelTranscription factor nuclear factorCytokine-induced NF-kappaB activationCell-permeable NBD peptideInhibitor of kappaBNF-κB activationNF-kappaB activityNF-kappaB activationAssociation of NEMOIKK complexAcute inflammationDevelopment of drugsProinflammatory activationInflammatory responseNBD peptideMouse modelProinflammatory stimuliIκB kinase (IKK) complexNuclear factorRegulatory protein NEMOInflammationSelective inhibitionExpression of genesInterferon-γ elicits arteriosclerosis in the absence of leukocytes
Tellides G, Tereb D, Kirkiles-Smith N, Kim R, Wilson J, Schechner J, Lorber M, Pober J. Interferon-γ elicits arteriosclerosis in the absence of leukocytes. Nature 2000, 403: 207-211. PMID: 10646607, DOI: 10.1038/35003221.Peer-Reviewed Original ResearchMeSH KeywordsAdultAnimalsArteriosclerosisCell DivisionCells, CulturedCoronary VesselsHistocompatibility AntigensHumansImage Processing, Computer-AssistedImmunohistochemistryInterferon-gammaLeukocytesMiceMice, SCIDMuscle, Smooth, VascularPlatelet-Derived Growth FactorReceptor, Platelet-Derived Growth Factor betaSwineTransplantation, HeterologousConceptsVascular smooth muscle cellsGraft arteriosclerosisIntimal expansionAbsence of leukocytesLesions of atherosclerosisSmooth muscle cellsAllogeneic transplantationArteriosclerotic changesAtheroma formationCytokine interferonExogenous IFNAntigen presentationT cellsImmunodeficient miceMononuclear leukocytesMouse modelArterial intimaIFNMuscle cellsArteriosclerosisLeukocytesHuman arteriesAtherosclerosisCellsTransplantation