2022
Assembly and architecture of the type III secretion sorting platform
Soto J, Galán J, Lara-Tejero M. Assembly and architecture of the type III secretion sorting platform. Proceedings Of The National Academy Of Sciences Of The United States Of America 2022, 119: e2218010119. PMID: 36512499, PMCID: PMC9907115, DOI: 10.1073/pnas.2218010119.Peer-Reviewed Original ResearchConceptsType III secretion machinesType III secretion systemTarget eukaryotic cellsType III secretionSecretion of proteinsBacterial nanomachinesSecretion machineEukaryotic cellsExport pathwayImportant bacterial pathogensSecretion systemBacterial structureAntivirulence strategiesCoordinated mechanismFunctional complexityBacterial pathogensGenetic deletionStructure modelingProtein deliveryAssemblyRational developmentCross-linking strategyAssembly processProteinDeletion
2008
Modulation of the actin cytoskeleton by Salmonella
Galan J. Modulation of the actin cytoskeleton by Salmonella. The FASEB Journal 2008, 22: 530.1-530.1. DOI: 10.1096/fasebj.22.1_supplement.530.1.Peer-Reviewed Original ResearchEffector proteinsHost cellsActin cytoskeletonBacterial proteinsCellular responsesBacterial effector proteinsProtein secretion systemSpecialized protein secretion systemActin cytoskeletal reorganizationDownstream effector proteinsSignal transduction pathwaysProtein Cdc42Exchange factorMembrane rufflingSmall GTPRho GTPasesSecretion systemCytoskeletal reorganizationTransduction pathwaysCytoskeletal rearrangementsCdc42RhoG.ProteinCytoskeletonRac
2003
A Salmonella protein causes macrophage cell death by inducing autophagy
Hernandez LD, Pypaert M, Flavell RA, Galán J. A Salmonella protein causes macrophage cell death by inducing autophagy. Journal Of Cell Biology 2003, 163: 1123-1131. PMID: 14662750, PMCID: PMC2173598, DOI: 10.1083/jcb.200309161.Peer-Reviewed Original ResearchConceptsMembrane fusion activityCell deathType III protein secretion systemFusion activityProtein secretion systemMacrophage cell deathEndoplasmic reticulum markerSalmonella-infected macrophagesSecretion systemSalmonella proteinsAutophagic vesiclesMutant formsSipBHost cellsCultured cellsMitochondriaMembrane structureSalmonella entericaProteinCausative agentMarked accumulationMacrophagesCellsAutophagosomesAutophagy
2001
Role of tyrosine kinases and the tyrosine phosphatase SptP in the interaction of Salmonella with host cells
Murli S, Watson R, Galán J. Role of tyrosine kinases and the tyrosine phosphatase SptP in the interaction of Salmonella with host cells. Cellular Microbiology 2001, 3: 795-810. PMID: 11736992, DOI: 10.1046/j.1462-5822.2001.00158.x.Peer-Reviewed Original ResearchMeSH KeywordsActinsBacterial ProteinsCdc42 GTP-Binding ProteinCell NucleusCytoskeletonInterleukin-8Mitogen-Activated Protein Kinase 1Mitogen-Activated Protein Kinase 3Mitogen-Activated Protein KinasesPhosphorylationPhosphotyrosineProtein Tyrosine PhosphatasesProtein-Tyrosine KinasesSalmonellaSalmonella typhimuriumSignal TransductionSubstrate SpecificityTransfectionTyrosineVimentinConceptsTyrosine phosphatase activityBacterial proteinsCellular responsesPhosphatase activityTyrosine kinaseHost cellsType III secretion systemIntermediate filament protein vimentinCarboxy-terminal domainRho family GTPases Cdc42Actin cytoskeleton reorganizationCellular transcription factorsMAP kinase activationInteraction of SalmonellaMembrane rufflesGAP activitySpecialized organellesGTPases Cdc42Secretion systemTranscription factorsCytoskeleton reorganizationKinase activationProtein vimentinBacterial uptakeProteinSALMONELLA INTERACTIONS WITH HOST CELLS: Type III Secretion at Work
Galán J. SALMONELLA INTERACTIONS WITH HOST CELLS: Type III Secretion at Work. Annual Review Of Cell And Developmental Biology 2001, 17: 53-86. PMID: 11687484, DOI: 10.1146/annurev.cellbio.17.1.53.Peer-Reviewed Original ResearchConceptsBacterial proteinsBacterial pathogen Salmonella entericaHost cellsType III secretion systemActin cytoskeleton dynamicsType III secretionHost cell functionsPathogen Salmonella entericaMolecular tinkeringConvergent evolutionEndocytic traffickingCytoskeleton dynamicsSpecialized organellesCellular functionsSecretion systemMicrobial pathogenesisRemarkable pathogenHost proteinsSalmonella interactionsVertebrate hostsProteinRemarkable arrayCoordinated actionCell functionSalmonella entericaSalmonella entry into host cells: the work in concert of type III secreted effector proteins
Zhou D, Galán J. Salmonella entry into host cells: the work in concert of type III secreted effector proteins. Microbes And Infection 2001, 3: 1293-1298. PMID: 11755417, DOI: 10.1016/s1286-4579(01)01489-7.Peer-Reviewed Original ResearchConceptsActin cytoskeleton rearrangementCytoskeleton rearrangementActin dynamicsHost cellsHost actin dynamicsHost signal transductionType III secretion systemActin-binding proteinsSPI-1 type III secretion systemEffector proteinsSecretion systemSignal transductionActin rearrangementBacterial proteinsSalmonella entryIntestinal epithelial cellsBacterial uptakeCdc42Coordinated stepsProteinEpithelial cellsRacRearrangementCellsTransductionStructural mimicry in bacterial virulence
Stebbins C, Galán J. Structural mimicry in bacterial virulence. Nature 2001, 412: 701-705. PMID: 11507631, DOI: 10.1038/35089000.Peer-Reviewed Original ResearchConceptsAmino acid sequence similarityConvergent evolutionCellular functionsSequence similarityDirect homologuesBacterial virulenceHost proteinsNew effectorsMicrobial pathogensFunctional mimicryStructural mimicryMolecular levelVirulence factorsMimicryStructural studiesHost factorsImportant mechanismHost activityHomologuesVirulenceEffectorsProteinPathogensSimilarityCommon and Contrasting Themes of Plant and Animal Diseases
Staskawicz B, Mudgett M, Dangl J, Galan J. Common and Contrasting Themes of Plant and Animal Diseases. Science 2001, 292: 2285-2289. PMID: 11423652, DOI: 10.1126/science.1062013.Peer-Reviewed Original ResearchConceptsHost-pathogen interactionsStudy of plantsDefense signalingHost defense responsesAnimal diseasesDefense responsesEffector proteinsAnimal pathogenesisBacterial pathogenesisPathogen virulencePathogen recognitionMolecular eventsHost resistancePlantsRecent studiesBacterial colonizationSignalingVirulenceProteinColonizationHostMechanismFuture studiesPathogenesis
2000
Biophysical characterization of SipA, an actin‐binding protein from Salmonella enterica
Mitra K, Zhou D, Galán J. Biophysical characterization of SipA, an actin‐binding protein from Salmonella enterica. FEBS Letters 2000, 482: 81-84. PMID: 11018527, DOI: 10.1016/s0014-5793(00)02040-8.Peer-Reviewed Original ResearchConceptsActin dynamicsCoiled-coil domainF-actin stabilityActin-binding proteinsActin cytoskeleton reorganizationNon-phagocytic cellsBacterial entryCytoskeleton reorganizationAdjacent actin monomersC-terminal fragmentBiophysical characterizationF-actinCellular responsesHost cellsBacterial uptakeActin monomersProteinSalmonella entericaHelical conformationSalmonella enterica infectionsIntestinal epitheliumEnterica infectionsEssential stepSIPACells
1999
A Salmonella protein antagonizes Rac-1 and Cdc42 to mediate host-cell recovery after bacterial invasion
Fu Y, Galán J. A Salmonella protein antagonizes Rac-1 and Cdc42 to mediate host-cell recovery after bacterial invasion. Nature 1999, 401: 293-297. PMID: 10499590, DOI: 10.1038/45829.Peer-Reviewed Original ResearchMeSH KeywordsActinsArginineBacterial AdhesionBacterial ProteinsCdc42 GTP-Binding Protein, Saccharomyces cerevisiaeCell Cycle ProteinsCell MembraneEscherichia coliGTP-Binding ProteinsGTPase-Activating ProteinsHumansJNK Mitogen-Activated Protein KinasesMAP Kinase Kinase 4Mitogen-Activated Protein Kinase KinasesMutationProtein KinasesProtein Tyrosine PhosphatasesProteinsRecombinant Fusion ProteinsSalmonella typhimuriumConceptsHost cell cytosolActin cytoskeletonType III secretion systemProtein secretion systemSpecialized protein secretion systemActin cytoskeleton reorganizationCell actin cytoskeletonActin cytoskeletal changesRho GTPase proteinsRac-1Bacterial effectorsEffector proteinsExchange factorGTPase proteinsSecretion systemSalmonella proteinsCytoskeletal changesCellular responsesCdc42ProteinInfected cellsBacterial invasionCytosolBacteriumSPTPAn invasion-associated Salmonella protein modulates the actin-bundling activity of plastin
Zhou D, Mooseker M, Galán J. An invasion-associated Salmonella protein modulates the actin-bundling activity of plastin. Proceedings Of The National Academy Of Sciences Of The United States Of America 1999, 96: 10176-10181. PMID: 10468582, PMCID: PMC17862, DOI: 10.1073/pnas.96.18.10176.Peer-Reviewed Original ResearchConceptsActin-bundling activityT-plastinBacterial-host cell contactHost cell signal transduction pathwaysHost cellsType III secretion systemBacterial effector proteinsActin-binding proteinsSignal transduction pathwaysBacterial effectorsMembrane rufflesEffector proteinsActin cytoskeletonMembrane rufflingSecretion systemSalmonella proteinsBacterial entryBacterial internalizationSalmonella entrySignaling processesActin filamentsF-actinNonphagocytic cellsProteinCell contactDifferential Regulation of Salmonella typhimurium Type III Secreted Proteins by Pathogenicity Island 1 (SPI-1)-Encoded Transcriptional Activators InvF and HilA
Eichelberg K, Galán J. Differential Regulation of Salmonella typhimurium Type III Secreted Proteins by Pathogenicity Island 1 (SPI-1)-Encoded Transcriptional Activators InvF and HilA. Infection And Immunity 1999, 67: 4099-4105. PMID: 10417179, PMCID: PMC96710, DOI: 10.1128/iai.67.8.4099-4105.1999.Peer-Reviewed Original ResearchConceptsExpression of componentsSPI-1Secretion systemRegulatory proteinsType III protein secretion systemType III secretion machineryCellular responsesHost cellsFunction mutationsType III secretion systemInvasion-associated type III secretion systemProtein secretion systemTranscriptional regulatory proteinsComplex regulatory mechanismsPathogenicity island 1Secretion machineryCentisome 63Different genesRegulatory mechanismsMutant strainPathogenicity islandInvFIsland 1Differential regulationProteinType III Secretion Machines: Bacterial Devices for Protein Delivery into Host Cells
Galán J, Collmer A. Type III Secretion Machines: Bacterial Devices for Protein Delivery into Host Cells. Science 1999, 284: 1322-1328. PMID: 10334981, DOI: 10.1126/science.284.5418.1322.Peer-Reviewed Original ResearchConceptsSecretion systemComplex protein secretion systemsHost cellsType III secretion systemBacterial effector proteinsProtein secretion systemAnimal pathogenic bacteriaHost cellular functionsGram-negative pathogenic bacteriaBacterial devicesPathogenic bacteriaEffector proteinsCellular functionsFlagellar apparatusBacterial speciesEffector moleculesProtein deliveryBacteriaCellsNovel preventionBiologySpeciesPlantsProteinTherapeutic approachesInteraction of Salmonella spp. with host cells
Murli S, Galán J. Interaction of Salmonella spp. with host cells. Advances In Cellular And Molecular Biology Of Membranes And Organelles 1999, 6: 173-180. DOI: 10.1016/s1874-5172(99)80011-x.Peer-Reviewed Original ResearchEffector proteinsHost cellsBacterial effector proteinsHost cell cytosolSignal transduction pathwaysHost cell responsesFacultative intracellular pathogenActin cytoskeletonMembrane rufflingTranscription factorsTransduction pathwaysElaborate mechanismsCell cytosolSubsequent translocationMicrobial pathogensCell deathHost environmentCell typesIntracellular pathogensDistinct responsesSalmonella entericaPathogensHostProteinIntimate interaction
1998
Identification of a Specific Chaperone for SptP, a Substrate of the Centisome 63 Type III Secretion System ofSalmonella typhimurium
Fu Y, Galán J. Identification of a Specific Chaperone for SptP, a Substrate of the Centisome 63 Type III Secretion System ofSalmonella typhimurium. Journal Of Bacteriology 1998, 180: 3393-3399. PMID: 9642193, PMCID: PMC107295, DOI: 10.1128/jb.180.13.3393-3399.1998.Peer-Reviewed Original ResearchMeSH KeywordsActinsAmino Acid SequenceBacterial ProteinsBase SequenceChromosomes, BacterialCloning, MolecularComplement Inactivator ProteinsCytoskeletonKineticsMolecular Sequence DataPlasmidsPolymerase Chain ReactionProtein Tyrosine PhosphatasesRecombinant Fusion ProteinsSalmonella typhimuriumSequence AlignmentSequence Homology, Amino AcidConceptsType III secretion systemSecretion systemSpecific chaperonesActin cytoskeletonTyrosine phosphataseType III protein secretion systemHost cell actin cytoskeletonHost cellsProtein secretion systemCell actin cytoskeletonHost cell responsesPulse-chase experimentsCytoplasmic chaperonesCentisome 63Effector proteinsTranscription factorsAcidic polypeptidesChaperonesResidues 15Function mutationsEffector moleculesCytoskeletonProteinSPTPSalmonella typhimuriumS. typhimurium Encodes an Activator of Rho GTPases that Induces Membrane Ruffling and Nuclear Responses in Host Cells
Hardt W, Chen L, Schuebel K, Bustelo X, Galán J. S. typhimurium Encodes an Activator of Rho GTPases that Induces Membrane Ruffling and Nuclear Responses in Host Cells. Cell 1998, 93: 815-826. PMID: 9630225, DOI: 10.1016/s0092-8674(00)81442-7.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBacterial ProteinsCalcium-Calmodulin-Dependent Protein KinasesCdc42 GTP-Binding ProteinCell Cycle ProteinsCell MembraneCell NucleusCOS CellsCytoskeletonEnzyme ActivationGTP PhosphohydrolasesGTP-Binding ProteinsGTPase-Activating ProteinsGuanosine DiphosphateGuanosine TriphosphateHeLa CellsHumansJNK Mitogen-Activated Protein KinasesMitogen-Activated Protein KinasesProteinsSalmonella typhimuriumSignal TransductionConceptsHost cellsMembrane rufflingSecretion systemGDP/GTP nucleotide exchangeProtein secretion systemActivator of RhoCDNA library screenActin cytoskeleton rearrangementRac-1Rho GTPasesCytoskeletal reorganizationCytoskeleton rearrangementBacterial proteinsNucleotide exchangeLibrary screenJNK activationCellular responsesNuclear responseCdc42Pathogen inducesSopERufflingMicrobial stimulationProteinCellsThe Salmonella typhimurium tyrosine phosphatase SptP is translocated into host cells and disrupts the actin cytoskeleton
Fu Y, Galán J. The Salmonella typhimurium tyrosine phosphatase SptP is translocated into host cells and disrupts the actin cytoskeleton. Molecular Microbiology 1998, 27: 359-368. PMID: 9484891, DOI: 10.1046/j.1365-2958.1998.00684.x.Peer-Reviewed Original ResearchConceptsActin cytoskeletonHost cell actin cytoskeletonFusion proteinHost cellsAmino acidsAmino-terminal halfCell actin cytoskeletonCultured cells resultsWild-type toxinCultured epithelial cellsEffector proteinsModular domainsStress fibersActive GSTCytoskeletonProteinCells resultsEpithelial cellsGSTSPTPProlonged incubationTranslocationCellsS. typhimuriumMicroinjection
1997
A secreted Salmonella protein with homology to an avirulence determinant of plant pathogenic bacteria
Hardt W, Galán J. A secreted Salmonella protein with homology to an avirulence determinant of plant pathogenic bacteria. Proceedings Of The National Academy Of Sciences Of The United States Of America 1997, 94: 9887-9892. PMID: 9275221, PMCID: PMC23287, DOI: 10.1073/pnas.94.18.9887.Peer-Reviewed Original ResearchConceptsPlant pathogenic bacteriaType III protein secretion systemProtein secretion systemSecretion systemPlant pathogen Xanthomonas campestris pvPathogen Xanthomonas campestris pvType III secretion systemPathogenic bacteriaXanthomonas campestris pvAvirulence determinantEffector proteinsSequence similaritySalmonella proteinsBacterial proteinsCampestris pvPutative targetsSophisticated mechanismsHost cellsYersinia pseudotuberculosisNovel familyProteinBacterial pathogensNovel targetSalmonella entericaBacteriaThe invasion-associated type-III protein secretion system inSalmonella – a review1Presented at the Workshop on `Type-4 Pili – Biogenesis, Adhesins, Protein Export, and DNA Import', Schloss Ringberg, Germany, 26–29 November 1995.1
Collazo C, Galán J. The invasion-associated type-III protein secretion system inSalmonella – a review1Presented at the Workshop on `Type-4 Pili – Biogenesis, Adhesins, Protein Export, and DNA Import', Schloss Ringberg, Germany, 26–29 November 1995.1. Gene 1997, 192: 51-59. PMID: 9224874, DOI: 10.1016/s0378-1119(96)00825-6.Peer-Reviewed Original ResearchConceptsProtein secretion systemSecretion systemType-4 Pili – BiogenesisHost cellsEukaryotic host cellsSpecialized protein secretion systemNon-phagocytic cellsMolecular genetic analysisDNA importPili biogenesisProtein exportSchloss RingbergCentisome 63Bacterial chromosomeAnimal pathogensGenetic analysisPathogenicity islandGenetic determinantsCellsReview1PresentedChromosomesPlantsProteinTranslocationAdhesinsThe invasion‐associated type III system of Salmonella typhimurium directs the translocation of Sip proteins into the host cell
Collazo C, Galán J. The invasion‐associated type III system of Salmonella typhimurium directs the translocation of Sip proteins into the host cell. Molecular Microbiology 1997, 24: 747-756. PMID: 9194702, DOI: 10.1046/j.1365-2958.1997.3781740.x.Peer-Reviewed Original ResearchConceptsProtein secretion systemProtein translocationHost cellsSIP proteinHenle-407 cellsType III protein secretion systemType III secretion systemType III secretion apparatusType III systemSubset of targetsCentisome 63Secretion apparatusSecretion systemBacterial entryBacterial internalizationNull mutationSalmonella typhimuriumCytochalasin DTranslocation processTranslocationProteinMutationsPresence of gentamicinSipBS. typhimurium strain