2020
A Static Self-Directed Method for Generating Brain Organoids from Human Embryonic Stem Cells.
Boisvert EM, Means RE, Michaud M, Thomson JJ, Madri JA, Katz SG. A Static Self-Directed Method for Generating Brain Organoids from Human Embryonic Stem Cells. Journal Of Visualized Experiments 2020 PMID: 32202516, PMCID: PMC7245934, DOI: 10.3791/60379.Peer-Reviewed Original ResearchConceptsEmbryonic stem cellsCell typesStem cellsIntrinsic developmental cuesHuman embryonic stem cellsHuman pluripotent stem cellsBrain organoidsBrain cell typesPluripotent stem cellsBasement membrane matrixMultiple cell typesDevelopmental cuesUse of organoidsExogenous growth factorsQuantitative reverse transcription polymerase chain reactionMultitude of diseasesHuman brain organoidsOrganoid growthSingle cellsReal-time quantitative reverse transcription polymerase chain reactionSpatial organizationOrganoidsGenetic disordersGrowth factorReverse transcription-polymerase chain reaction
1993
Spatial organization of the extracellular matrix modulates the expression of PDGF-receptor subunits in mesangial cells
Marx M, Daniel T, Kashgarian M, Madri J. Spatial organization of the extracellular matrix modulates the expression of PDGF-receptor subunits in mesangial cells. Kidney International 1993, 43: 1027-1041. PMID: 8510381, DOI: 10.1038/ki.1993.145.Peer-Reviewed Original ResearchConceptsPDGF receptor subunitsMesangial cell phenotypeCell surfaceExtracellular matrixCell phenotypeExtracellular matrix environmentSpatial organizationPDGF responsivenessPDGF receptor alphaTwo-dimensional cultureMulticellular structuresThree-dimensional cultureMetabolic labelingDifferential expressionQuiescent cellsRat mesangial cell growthCell growthPDGF isoforms AASubunitsMatrix environmentCell proliferationPDGF isoformsReceptor subunitsUltrastructural analysisSurface expression