2020
A Static Self-Directed Method for Generating Brain Organoids from Human Embryonic Stem Cells.
Boisvert EM, Means RE, Michaud M, Thomson JJ, Madri JA, Katz SG. A Static Self-Directed Method for Generating Brain Organoids from Human Embryonic Stem Cells. Journal Of Visualized Experiments 2020 PMID: 32202516, PMCID: PMC7245934, DOI: 10.3791/60379.Peer-Reviewed Original ResearchMeSH KeywordsBrainCell DifferentiationHuman Embryonic Stem CellsHumansMesencephalonNeuronsOrganoidsPluripotent Stem CellsTissue Culture TechniquesConceptsEmbryonic stem cellsCell typesStem cellsIntrinsic developmental cuesHuman embryonic stem cellsHuman pluripotent stem cellsBrain organoidsBrain cell typesPluripotent stem cellsBasement membrane matrixMultiple cell typesDevelopmental cuesUse of organoidsExogenous growth factorsQuantitative reverse transcription polymerase chain reactionMultitude of diseasesHuman brain organoidsOrganoid growthSingle cellsReal-time quantitative reverse transcription polymerase chain reactionSpatial organizationOrganoidsGenetic disordersGrowth factorReverse transcription-polymerase chain reaction
2011
GSK-3β: a signaling pathway node modulating neural stem cell and endothelial cell interactions
Li Q, Michaud M, Canosa S, Kuo A, Madri JA. GSK-3β: a signaling pathway node modulating neural stem cell and endothelial cell interactions. Angiogenesis 2011, 14: 173-185. PMID: 21253820, DOI: 10.1007/s10456-011-9201-9.Peer-Reviewed Original ResearchMeSH KeywordsAminophenolsAnimalsBasic Helix-Loop-Helix Transcription FactorsBeta CateninBrainCell CommunicationCell DifferentiationCell MovementCell ProliferationEndothelial CellsEnzyme ActivationGlycogen Synthase Kinase 3Glycogen Synthase Kinase 3 betaHypoxia-Inducible Factor 1, alpha SubunitIntercellular Signaling Peptides and ProteinsMaleMaleimidesMiceMice, Inbred C57BLNeovascularization, PhysiologicNeural Stem CellsNeurogenesisPhosphorylationPhosphoserineReceptor Cross-TalkSignal TransductionSolubilitySpecies SpecificityConceptsNeural stem cellsNotch-1 expressionHIF-1αGSK-3βSDF-1III-tubulinStem cellsPremature infant populationMicrovascular endothelial cellsGSK-3β activationCD1 levelsEndothelial cell interactionsNeurogenic areasVascular proliferationInfant populationGSK-3β inhibitorTherapeutic potentialSVZ tissueGreater angiogenesisHIF-2αMouse strainsΒ-catenin participatesEndothelial cellsReciprocal modulation
2009
Strain Differences in Behavioral and Cellular Responses to Perinatal Hypoxia and Relationships to Neural Stem Cell Survival and Self-Renewal Modeling the Neurovascular Niche
Li Q, Liu J, Michaud M, Schwartz ML, Madri JA. Strain Differences in Behavioral and Cellular Responses to Perinatal Hypoxia and Relationships to Neural Stem Cell Survival and Self-Renewal Modeling the Neurovascular Niche. American Journal Of Pathology 2009, 175: 2133-2145. PMID: 19815710, PMCID: PMC2774076, DOI: 10.2353/ajpath.2009.090354.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBehavior, AnimalCell DifferentiationCell MovementCell SurvivalCells, CulturedChemokine CXCL12Endothelial CellsEnzyme ActivationFemaleHumansHypoxiaHypoxia-Inducible Factor 1, alpha SubunitHypoxia-Inducible Factor-Proline DioxygenasesInfantInfant, NewbornInfant, PrematureMaleMiceMice, Inbred C57BLMice, Inbred StrainsNeuronsNeuropsychological TestsPhosphatidylinositol 3-KinasesProcollagen-Proline DioxygenaseProto-Oncogene Proteins c-aktSignal TransductionStem CellsConceptsChronic hypoxiaC57 miceHIF-1alphaLow birth weight infant populationMatrix metalloproteinase-9 activityStromal-derived factor-1CD-1 miceMetalloproteinase-9 activityAdult C57 miceHypoxia-induced factorNeural stem cell survivalHigher apoptosis ratePerinatal hypoxiaRepair/recoveryClinical improvementNeurodevelopmental handicapPreventive therapyPremature infantsNeurogenic zonesNeurovascular nicheInfant populationC57BL/6 pupsProlyl hydroxylase domain 2Migratory responsivenessStem cell survivalBone Marrow Monocyte PECAM-1 Deficiency Elicits Increased Osteoclastogenesis Resulting in Trabecular Bone Loss
Wu Y, Tworkoski K, Michaud M, Madri JA. Bone Marrow Monocyte PECAM-1 Deficiency Elicits Increased Osteoclastogenesis Resulting in Trabecular Bone Loss. The Journal Of Immunology 2009, 182: 2672-2679. PMID: 19234161, DOI: 10.4049/jimmunol.0802398.Peer-Reviewed Original ResearchMeSH KeywordsAgingAnimalsBone Marrow CellsBone ResorptionCell DifferentiationCells, CulturedDown-RegulationFemaleIntracellular Signaling Peptides and ProteinsMiceMice, KnockoutMonocytesOsteoclastsOsteogenesisPhosphorylationPlatelet Endothelial Cell Adhesion Molecule-1Protein Tyrosine Phosphatase, Non-Receptor Type 6Protein-Tyrosine KinasesSyk KinaseZAP-70 Protein-Tyrosine KinaseConceptsOsteoclast-like cellsKO miceBone marrowPECAM-1-null miceTrabecular bone lossPECAM-1Trabecular bone volumeSize of osteoclastsNF-kappaB ligandOsteoclast precursor culturesSHP-1 interactionsNumber of trabeculaeWT miceBM monocytesBone lossBone resorptionReceptor activatorBone volumeSHP-1Precursor culturesNull miceMiceLong bonesSyk kinaseFurther studies
2004
A null mutation of Hhex results in abnormal cardiac development,defective vasculogenesis and elevated Vegfa levels
Hallaq H, Pinter E, Enciso J, McGrath J, Zeiss C, Brueckner M, Madri J, Jacobs HC, Wilson CM, Vasavada H, Jiang X, Bogue CW. A null mutation of Hhex results in abnormal cardiac development,defective vasculogenesis and elevated Vegfa levels. Development 2004, 131: 5197-5209. PMID: 15459110, DOI: 10.1242/dev.01393.Peer-Reviewed Original ResearchConceptsEpithelial-mesenchymal transformationVEGFA levelsVentricular septal defectVascular endothelial growth factorDefective vasculogenesisEndothelial growth factorEndocardial cushionsInhibitor of VEGFVascular developmentTract abnormalitiesSeptal defectSFlt-1Right ventricleNormal liverVentral foregut endodermNormal cardiovascular developmentReceptor 1Abnormal cardiac developmentGrowth factorNull mutationVentral foregutAberrant developmentCompact myocardiumAV explantsE8.5-9.0
2002
Disrupted synaptic development in the hypoxic newborn brain
Curristin SM, Cao A, Stewart WB, Zhang H, Madri JA, Morrow JS, Ment LR. Disrupted synaptic development in the hypoxic newborn brain. Proceedings Of The National Academy Of Sciences Of The United States Of America 2002, 99: 15729-15734. PMID: 12438650, PMCID: PMC137784, DOI: 10.1073/pnas.232568799.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAnimals, NewbornApoptosisAtmosphere Exposure ChambersBrain Damage, ChronicCell DifferentiationCytoskeletonDisease Models, AnimalDNA, ComplementaryEndothelial Growth FactorsGene Expression ProfilingHypoxiaHypoxia, BrainHypoxia-Inducible Factor 1, alpha SubunitIntercellular Signaling Peptides and ProteinsLymphokinesMembrane ProteinsMiceMice, Inbred C57BLMicrotubulesNerve Tissue ProteinsOligodendrogliaOligonucleotide Array Sequence AnalysisStress, PhysiologicalSynapsesSynaptic TransmissionTranscription FactorsTranscription, GeneticVascular Endothelial Growth Factor AVascular Endothelial Growth FactorsConceptsPostnatal hypoxiaCerebral maturationGlial maturationNewborn brainSynaptic maturationPresynaptic functionPostsynaptic functionSublethal hypoxiaSynaptic developmentHealth crisisHypoxiaCognitive disabilitiesBrainMaturation programMaturationDysynchronyNeuropathologyInfantsNeurotransmissionCohortProtein assaysMiceHypoxic
1997
An in vitro three-dimensional coculture model of cerebral microvascular angiogenesis and differentiation
Ment L, Stewart W, Scaramuzzino D, Madri J. An in vitro three-dimensional coculture model of cerebral microvascular angiogenesis and differentiation. In Vitro Cellular & Developmental Biology - Animal 1997, 33: 684-691. PMID: 9358284, DOI: 10.1007/s11626-997-0126-y.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAnimals, NewbornAstrocytesBrainCell DifferentiationCoculture TechniquesDogsEndothelium, VascularEnzyme ActivationFibronectinsImmunohistochemistryLamininMicrocirculationMicroscopy, ConfocalMicroscopy, FluorescenceModels, BiologicalNeovascularization, PhysiologicRatsRNA, MessengerUrokinase-Type Plasminogen ActivatorConceptsAstrocyte coculturesThree-dimensional cocultureBrain microvascular endothelial cellsNewborn beagle pupsPostnatal day 1Microvascular endothelial cellsNeonatal rat forebrainCell typesPlasminogen activator activityPreterm birthMicrovascular responsesBeagle pupsThree-dimensional coculture modelDay 1Rat forebrainGlial processesEndothelial proliferationMicrovascular angiogenesisEndothelial cellsCoculture modelPlasminogen zymographyOnly low levelsExtracellular matrix componentsTube formationCocultureNitric oxide synthase inhibitors attenuate transforming-growth-factor-beta 1-stimulated capillary organization in vitro.
Papapetropoulos A, Desai KM, Rudic RD, Mayer B, Zhang R, Ruiz-Torres MP, García-Cardeña G, Madri JA, Sessa WC. Nitric oxide synthase inhibitors attenuate transforming-growth-factor-beta 1-stimulated capillary organization in vitro. American Journal Of Pathology 1997, 150: 1835-44. PMID: 9137106, PMCID: PMC1858220.Peer-Reviewed Original ResearchConceptsNitric oxideL-NAMETube formationNOS isoformsNitric oxide synthase inhibitorL-nitro-arginine methylesterOxide synthase inhibitorNO donor sodium nitroprussideRole of NOTranscriptase-polymerase chain reactionExcess L-arginineDonor sodium nitroprussideSoluble guanylate cyclaseWestern blot analysisEndothelial cell proliferationNOS blockadeCapillary tube formationEndothelial NOSSodium nitroprussideSynthase inhibitorL-arginineMicrovascular ECsAutocrine productionGuanylate cyclaseCapillary organization
1996
Laminin promotes differentiation of NB4 promyelocytic leukemia cells with all-trans retinoic acid.
Becker P, Li Z, Potselueva T, Madri J, Newburger P, Berliner N. Laminin promotes differentiation of NB4 promyelocytic leukemia cells with all-trans retinoic acid. Blood 1996, 88: 261-7. PMID: 8704182, DOI: 10.1182/blood.v88.1.261.bloodjournal881261.Peer-Reviewed Original ResearchMeSH KeywordsAntigens, CDBase SequenceCalciumCell Culture TechniquesCell DifferentiationCell DivisionCollagenDrug SynergismFibronectinsGene Expression Regulation, LeukemicHumansIntegrin alpha6Integrin beta1IonomycinIonophoresLamininLeukemia, Promyelocytic, AcuteMolecular Sequence DataNeoplasm ProteinsNeoplastic Stem CellsOxidation-ReductionPolymerase Chain ReactionReceptors, LamininTretinoinTumor Cells, CulturedConceptsAlpha 6 integrinTrans retinoic acidNB4 promyelocytic leukemia cellsPromyelocytic leukemia cellsMorphologic maturationLeukemia cellsRetinoic acidReverse transcription-polymerase chain reactionTranscription-polymerase chain reactionSecondary granule proteinsPresence of ionomycinPromyelocytic leukemia cell lineHistologic appearanceHours of exposureLeukemia cell linesMinimal maturationFlow cytometryHigh-level surface expressionDifferentiation agentsCollagen type INB4 cellsLaminin receptorATRAExtracellular matrix componentsCell proliferationRestitution at the cellular level: regulation of the migrating phenotype.
Basson M, Rashid Z, Turowski G, West A, Emenaker N, Sgambati S, Hong F, Perdikis D, Datta S, Madri J. Restitution at the cellular level: regulation of the migrating phenotype. The Yale Journal Of Biology And Medicine 1996, 69: 119-29. PMID: 9112743, PMCID: PMC2588988.Peer-Reviewed Original ResearchMeSH KeywordsActinsAlkaline PhosphataseAnimalsCell DifferentiationCell MovementCells, CulturedCollagenDipeptidyl-Peptidases and Tripeptidyl-PeptidasesEnterostomyEpidermal Growth FactorEpithelial CellsEpitheliumHumansHydrogen-Ion ConcentrationIntestinal MucosaIntestinesJejunumLamininMembrane ProteinsMicrovilliPentagastrinPeptide YYPeptidesPhosphoproteinsRatsRats, Sprague-DawleyZonula Occludens-1 ProteinConceptsIntestinal epithelial cellsCell matrix proteinsIntestinal epithelial differentiationIntestinal epithelial brush borderCaco-2 intestinal epithelial cellsCaco-2 migrationEpithelial cellsEpithelial brush borderRegulatory biologyHuman Caco-2 intestinal epithelial cellsSheet migrationCell biologyCell motilityMigratory stateMatrix proteinsDipeptidyl dipeptidaseCellular levelBrush borderCaco-2 cellsEpithelial differentiationCell linesBiologyProteinDifferentiationSpecific activity
1994
Glutamine modulates phenotype and stimulates proliferation in human colon cancer cell lines.
Turowski G, Rashid Z, Hong F, Madri J, Basson M. Glutamine modulates phenotype and stimulates proliferation in human colon cancer cell lines. Cancer Research 1994, 54: 5974-80. PMID: 7954430.Peer-Reviewed Original ResearchMeSH KeywordsAlkaline PhosphataseBeta-GalactosidaseCathepsin CCell AdhesionCell DifferentiationCell DivisionColonic NeoplasmsDipeptidyl-Peptidases and Tripeptidyl-PeptidasesDose-Response Relationship, DrugExtracellular Matrix ProteinsGlutamineHumansIntegrinsLactaseOligo-1,6-GlucosidasePhenotypeTumor Cells, CulturedConceptsMatrix proteinsHuman colon carcinoma cell lineColon carcinoma cell lineCell linesCarcinoma cell linesSurface expressionDigestive enzyme expressionGlutamine-free mediumCell-matrix interactionsColon cancer cell linesHuman colon cancer cell linesCaco-2 proliferationSerial cell countsIntegrin surface expressionSW620 cellsCancer cell linesDigestive enzymesProteinEnzyme expressionGlutamineSynthetic substratesAdherent cellsCathepsin CExpressionIntegrin expression
1992
Extracellular matrix-cell interactions: dynamic modulators of cell, tissue and organism structure and function.
Madri J, Basson M. Extracellular matrix-cell interactions: dynamic modulators of cell, tissue and organism structure and function. Laboratory Investigation 1992, 66: 519-21. PMID: 1573848.Peer-Reviewed Original ResearchMatrix composition, organization and soluble factors: Modulators of microvascular cell differentiation in vitro
Madri J, Marx M. Matrix composition, organization and soluble factors: Modulators of microvascular cell differentiation in vitro. Kidney International 1992, 41: 560-565. PMID: 1573829, DOI: 10.1038/ki.1992.82.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCell DifferentiationCulture TechniquesEndothelium, VascularExtracellular MatrixGlomerular MesangiumMicrocirculationPhenotypeConceptsCell typesStructure/functionMajor cell typesExtracellular matrix componentsVascular cell behaviorVascular smooth muscle cellsEndothelial cellsCell differentiationSoluble factorsCell behaviorExtracellular matrixNeighboring cellsMetabolic functionsDirect interactionSmooth muscle cellsMatrix componentsCell populationsMorphological organizationMuscle cellsMicrovascular endothelial cellsCulture modelCellsMesangial cell populationOrgan culture modelCell isolation
1991
Effects of soluble factors and extracellular matrix components on vascular cell behavior in vitro and in vivo: Models of de‐endothelialization and repair
Madri J, Marx M, Merwin J, Basson C, Prinz C, Bell L. Effects of soluble factors and extracellular matrix components on vascular cell behavior in vitro and in vivo: Models of de‐endothelialization and repair. Journal Of Cellular Biochemistry 1991, 45: 123-130. PMID: 1711525, DOI: 10.1002/jcb.240450202.Peer-Reviewed Original ResearchConceptsSoluble factorsEndothelial cellsVascular smooth muscle cellsCell populationsSite of injuryMicrovascular endothelial cellsSmooth muscle cellsVessel endothelial cellsEndothelial vascular cellsLarge vessel endothelial cellsVascular cell populationsCell typesIntimal thickeningDenudation injuryVascular cell typesArterial mediaSubsequent lumen formationDifferent cell populationsInjuryCell responsesMuscle cellsVascular cellsExtracellular matrixSoft tissueVascular cell behavior
1990
Interactions of Vascular Cells with Transforming Growth Factors‐βa
MADRI J, KOCHER O, MERWIN J, BELL L, TUCKER A, BASSON C. Interactions of Vascular Cells with Transforming Growth Factors‐βa. Annals Of The New York Academy Of Sciences 1990, 593: 243-258. PMID: 1695825, DOI: 10.1111/j.1749-6632.1990.tb16116.x.Peer-Reviewed Original Research
1988
Extracellular matrix specificity for the differentiation of capillary endothelial cells
Carley W, Milici A, Madri J. Extracellular matrix specificity for the differentiation of capillary endothelial cells. Experimental Cell Research 1988, 178: 426-434. PMID: 3049122, DOI: 10.1016/0014-4827(88)90411-9.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCell DifferentiationCell LineEndothelium, VascularExtracellular MatrixFluorescent Antibody TechniqueMicroscopy, ElectronConceptsExtracellular matrixCapillary endothelial cellsEndothelial cellsSpecific extracellular matrixEndothelial cell migrationVivo phenotypeDifferentiated phenotypeCell migrationCell typesCell growthCell dedifferentiationEndothelial cell growthMatrix componentsMembrane structurePhenotypeCellsExpressionCulture methodCytoplasmProteinGrowthMadinDifferentiationDedifferentiationGreater number
1983
Capillary endothelial cell cultures: phenotypic modulation by matrix components.
Madri J, Williams S. Capillary endothelial cell cultures: phenotypic modulation by matrix components. Journal Of Cell Biology 1983, 97: 153-165. PMID: 6190818, PMCID: PMC2112496, DOI: 10.1083/jcb.97.1.153.Peer-Reviewed Original Research