2023
Proteasomes: Isolation and Activity Assays
Li Y, Tomko R, Hochstrasser M. Proteasomes: Isolation and Activity Assays. Current Protocols 2023, 3: e717. PMID: 37026813, PMCID: PMC10337785, DOI: 10.1002/cpz1.717.Peer-Reviewed Original ResearchConceptsRegulatory particleOne-step purification schemeCore particlesMultisubunit protease complexUbiquitin-proteasome systemUbiquitin polypeptidesUnneeded proteinsYeast SaccharomycesProtein substratesProtease complexProteasomeGel filtration stepPurification schemeProteolytic activityEukaryotesSaccharomycesPolypeptideProteinSubstrateAssaysComplexes
1999
Structure and functional analyses of the 26S proteasome subunits from plants – Plant 26S proteasome
Fu H, Girod P, Doelling J, van Nocker S, Hochstrasser M, Finley D, Vierstra R. Structure and functional analyses of the 26S proteasome subunits from plants – Plant 26S proteasome. Molecular Biology Reports 1999, 26: 137-146. PMID: 10363660, DOI: 10.1023/a:1006926322501.Peer-Reviewed Original ResearchConceptsProteasome degrades ubiquitinated proteinsUbiquitin fusion degradation (UFD) pathwayStructure/function analysisAAA-ATPase subunitsC-terminal motifDegradation of proteinsRegulatory particle subunitsCore particlesPlant 26SRpn10 geneArabidopsis genesYeast counterpartMoss PhyscomitrellaArabidopsis thalianaPlant hormonesUbiquitin systemProteasome genesDevelopmental programHomologous recombinationProteolytic complexPlant subunitsReverse geneticsUbiquitinated proteinsUbiquitin conjugatesVivo function