2023
Proteasomes: Isolation and Activity Assays
Li Y, Tomko R, Hochstrasser M. Proteasomes: Isolation and Activity Assays. Current Protocols 2023, 3: e717. PMID: 37026813, PMCID: PMC10337785, DOI: 10.1002/cpz1.717.Peer-Reviewed Original ResearchConceptsRegulatory particleOne-step purification schemeCore particlesMultisubunit protease complexUbiquitin-proteasome systemUbiquitin polypeptidesUnneeded proteinsYeast SaccharomycesProtein substratesProtease complexProteasomeGel filtration stepPurification schemeProteolytic activityEukaryotesSaccharomycesPolypeptideProteinSubstrateAssaysComplexes
1998
The Deubiquitinating Enzymes
Wilkinson K, Hochstrasser M. The Deubiquitinating Enzymes. 1998, 99-125. DOI: 10.1007/978-1-4899-1922-9_4.Peer-Reviewed Original ResearchPolyubiquitin chainsTypes of ubiquitinationReceptor-mediated signal transductionCell cycle progressionModification of proteinsOrganelle biogenesisUbiquitin polypeptidesChromosome structureUbiquitin moleculesCellular processesProtein localizationSignal transductionDeubiquitinating enzymeIsopeptide linkageLysine 48Lysine 6C-terminusGene expressionIsopeptide bondsStress responseUbiquitinProteinProtein metabolismViral pathogenesisBiogenesis