Molecular Biology of Cytoplasmic Incompatibility Caused by Wolbachia Endosymbionts
Hochstrasser M. Molecular Biology of Cytoplasmic Incompatibility Caused by Wolbachia Endosymbionts. Annual Review Of Microbiology 2023, 77: 299-316. PMID: 37285552, DOI: 10.1146/annurev-micro-041020-024616.Peer-Reviewed Original ResearchConceptsCytoplasmic incompatibilityMale killingHost ubiquitin systemEndosymbiotic bacteriaFemale germlineEukaryotic cellsCognate partnersEmbryonic lethalityBicistronic operonUbiquitin systemDownstream genesWolbachia endosymbiontReproductive advantageCI inductionMolecular biologyInfected femalesEndosymbiontsInfections of malesReproductive alterationsDeubiquitylaseDeubiquitylasesOperonParthenogenesisArthropodsGermlineProteasomes: Isolation and Activity Assays
Li Y, Tomko R, Hochstrasser M. Proteasomes: Isolation and Activity Assays. Current Protocols 2023, 3: e717. PMID: 37026813, PMCID: PMC10337785, DOI: 10.1002/cpz1.717.Peer-Reviewed Original ResearchConceptsRegulatory particleOne-step purification schemeCore particlesMultisubunit protease complexUbiquitin-proteasome systemUbiquitin polypeptidesUnneeded proteinsYeast SaccharomycesProtein substratesProtease complexProteasomeGel filtration stepPurification schemeProteolytic activityEukaryotesSaccharomycesPolypeptideProteinSubstrateAssaysComplexes