2022
A discovery-based proteomics approach identifies protein disulphide isomerase (PDIA1) as a biomarker of β cell stress in type 1 diabetes
Syed F, Singhal D, Raedschelders K, Krishnan P, Bone R, McLaughlin M, Van Eyk J, Mirmira R, Yang M, Mamula M, Wu H, Liu X, Evans-Molina C. A discovery-based proteomics approach identifies protein disulphide isomerase (PDIA1) as a biomarker of β cell stress in type 1 diabetes. EBioMedicine 2022, 87: 104379. PMID: 36463755, PMCID: PMC9719098, DOI: 10.1016/j.ebiom.2022.104379.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBiomarkersChildDiabetes Mellitus, Type 1FemaleHumansInsulin-Secreting CellsIslets of LangerhansMiceMice, Inbred NODMice, SCIDProtein Disulfide-IsomerasesProteomicsConceptsT-cell adoptive transferCell adoptive transferNOD miceNOD-SCID miceType 1 diabetesΒ-cell stressAdoptive transferPre-diabetic NOD miceFemale NOD miceNon-diabetic controlsRecent-onset T1DSerum of childrenDistinct mouse modelsΒ-cell functionHuman organ donorsWeeks of agePotential human biomarkersDisease-related changesΒ-cell deathCell protein expressionNOD isletsAutoantibody positivityDiabetes onsetT1D developmentImmune activationBiomarkers of autoimmunity and beta cell metabolism in type 1 diabetes
Yang M, Kibbey R, Mamula M. Biomarkers of autoimmunity and beta cell metabolism in type 1 diabetes. Frontiers In Immunology 2022, 13: 1028130. PMID: 36389721, PMCID: PMC9647083, DOI: 10.3389/fimmu.2022.1028130.Peer-Reviewed Original ResearchMeSH KeywordsAutoimmune DiseasesAutoimmunityBiomarkersDiabetes Mellitus, Type 1HumansInsulin-Secreting CellsConceptsPosttranslational protein modificationMetabolic pathwaysType 1 diabetesCellular metabolic pathwaysImportant biological functionsAutoimmune diseasesBeta cellsCellular metabolic dysfunctionPancreatic isletsProtein modificationBiological functionsProtein structureInsulin-producing beta cellsBiomarkers of autoimmunityChronic autoimmune diseaseCell metabolismBeta-cell metabolismNumerous autoimmune diseasesPancreatic beta cellsPotential pathological consequencesNormal metabolic pathwaysDisease activityPathological consequencesSpecific autoantigensSpecific autoimmunity
2021
Gene expression signatures of target tissues in type 1 diabetes, lupus erythematosus, multiple sclerosis, and rheumatoid arthritis
Szymczak F, Colli M, Mamula M, Evans-Molina C, Eizirik D. Gene expression signatures of target tissues in type 1 diabetes, lupus erythematosus, multiple sclerosis, and rheumatoid arthritis. Science Advances 2021, 7: eabd7600. PMID: 33523973, PMCID: PMC7787485, DOI: 10.1126/sciadv.abd7600.Peer-Reviewed Original ResearchMeSH KeywordsArthritis, RheumatoidAutoimmune DiseasesDiabetes Mellitus, Type 1HumansLupus Erythematosus, SystemicMultiple SclerosisTranscriptomeConceptsType 1 diabetesLupus erythematosusTarget tissuesRheumatoid arthritisMultiple sclerosisAutoimmune diseasesImmune systemSystemic lupus erythematosusTarget tissue levelRepurposing of drugsDisease-specific signaturesGene expression signaturesImmune assaultSimilar molecular signaturesClinical useTissue levelsDiseaseHigh expressionExpression signaturesDifferent diseasesErythematosusArthritisSclerosisDiabetesTherapy
2019
Islet Autoantibody Standardization Program 2018 Workshop: Interlaboratory Comparison of Glutamic Acid Decarboxylase Autoantibody Assay Performance
Lampasona V, Pittman D, Williams A, Achenbach P, Schlosser M, Akolkar B, Winter W, Laboratories P, Watson K, Weets I, Tao Y, Chen V, Yang Y, Uibo R, Reimand K, Knip M, Härkönen T, Chatenoud L, Achenbach P, Neidhoefer S, Schlosser M, Lampasona V, Kawasaki E, Batstra M, Cieremans T, Almås B, Opsion K, Wyka K, Castaño L, Ramelius A, Johansson I, Williams A, Furmaniak J, McDonald T, McLaughlin K, Christie M, Metz A, Mathew A, Hampe C, Lu C, Wasserfall C, Mann C, Pittman D, Ananta J, Yu L, Mamula M, Robinson P, Gaur V, Hagopian W. Islet Autoantibody Standardization Program 2018 Workshop: Interlaboratory Comparison of Glutamic Acid Decarboxylase Autoantibody Assay Performance. Clinical Chemistry 2019, 65: 1141-1152. PMID: 31409598, PMCID: PMC8936135, DOI: 10.1373/clinchem.2019.304196.Peer-Reviewed Original Research