2015
Proteomics and Transcriptomics of BJAB Cells Expressing the Epstein-Barr Virus Noncoding RNAs EBER1 and EBER2
Pimienta G, Fok V, Haslip M, Nagy M, Takyar S, Steitz JA. Proteomics and Transcriptomics of BJAB Cells Expressing the Epstein-Barr Virus Noncoding RNAs EBER1 and EBER2. PLOS ONE 2015, 10: e0124638. PMID: 26121143, PMCID: PMC4487896, DOI: 10.1371/journal.pone.0124638.Peer-Reviewed Original ResearchConceptsMRNA-seq dataHost cell nucleusBJAB cellsCell proliferationGene expression featuresPro-survival effectsProtein adaptersAlternative splicingMRNA transcriptomeUpregulated proteinsSILAC dataRich elementsAkt activationPI3K-AktBiochemical assaysCell nucleiEBV latencySwitch eventsProteinMaintenance of latencyCell linesVEGFA proteinMechanistic explanationUpregulated oncogenesPIK3AP1
2005
mRNA Helicase Activity of the Ribosome
Takyar S, Hickerson RP, Noller HF. mRNA Helicase Activity of the Ribosome. Cell 2005, 120: 49-58. PMID: 15652481, DOI: 10.1016/j.cell.2004.11.042.Peer-Reviewed Original ResearchConceptsHelicase activitySecondary structureHelicase active siteMutation of residuesVitro translation systemMRNA secondary structureDownstream mRNA secondary structureAbility of ribosomesDownstream helicesProtein S3Most mRNAsBase-paired helicesRibosomesTranslation systemHelixActive siteDisplacement assayDownstream tunnelHelicaseCodonProcessivitySubunitsMutationsMRNAResidues