2023
AAV-mediated delivery of a Sleeping Beauty transposon and an mRNA-encoded transposase for the engineering of therapeutic immune cells
Ye L, Lam S, Yang L, Suzuki K, Zou Y, Lin Q, Zhang Y, Clark P, Peng L, Chen S. AAV-mediated delivery of a Sleeping Beauty transposon and an mRNA-encoded transposase for the engineering of therapeutic immune cells. Nature Biomedical Engineering 2023, 8: 132-148. PMID: 37430157, PMCID: PMC11320892, DOI: 10.1038/s41551-023-01058-6.Peer-Reviewed Original ResearchGene delivery systemsAdeno-associated virusTransgene expressionHigh transgene expressionSleeping Beauty (SB) transposonTransgene deliverySleeping Beauty transposasePluripotent stem cellsSB transposonLentiviral vectorsGenomic integrationEngineering cellsBeauty transposonMinicircle DNATransposon DNABeauty transposaseCell viabilityPermanent integrationStem cellsElectroporation of plasmidsTransgeneChimeric antigen receptorDeliveryElectroporationEngineering
2014
CRISPR-Cas9 Knockin Mice for Genome Editing and Cancer Modeling
Platt RJ, Chen S, Zhou Y, Yim MJ, Swiech L, Kempton HR, Dahlman JE, Parnas O, Eisenhaure TM, Jovanovic M, Graham DB, Jhunjhunwala S, Heidenreich M, Xavier RJ, Langer R, Anderson DG, Hacohen N, Regev A, Feng G, Sharp PA, Zhang F. CRISPR-Cas9 Knockin Mice for Genome Editing and Cancer Modeling. Cell 2014, 159: 440-455. PMID: 25263330, PMCID: PMC4265475, DOI: 10.1016/j.cell.2014.09.014.Peer-Reviewed Original Research