2022
Super-resolved fluorescence imaging of peripheral nerve
Hernández I, Mohan S, Minderler S, Jowett N. Super-resolved fluorescence imaging of peripheral nerve. Scientific Reports 2022, 12: 12450. PMID: 35864187, PMCID: PMC9304405, DOI: 10.1038/s41598-022-16769-0.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAxonsHumansMammalsMiceMicroscopy, ElectronMyelin SheathOptical ImagingSciatic NerveConceptsSuper-resolution microscopySuper-resolution imagingNanoscale resolutionSuper-resolution imaging techniquesEmission depletion (STED) microscopyNanoscale cellular structuresStructured illumination microscopyElectron microscopyVisible lightDiffraction limited resolutionDepletion microscopyMicroscopyFluorescence imagingNon-toxic meansIllumination microscopyBiomedical sciencesFluorescent dyeBrightfield microscopyFluorescent objectsSample preparationFluctuation microscopyAutomated stain-free histomorphometry of peripheral nerve by contrast-enhancing techniques and artificial intelligence
Coto Hernández I, Mohan S, Jowett N. Automated stain-free histomorphometry of peripheral nerve by contrast-enhancing techniques and artificial intelligence. Journal Of Neuroscience Methods 2022, 375: 109598. PMID: 35436515, DOI: 10.1016/j.jneumeth.2022.109598.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsArtificial IntelligenceAxonsColoring AgentsMammalsMiceMyelin SheathSciatic NerveConceptsDeep learning algorithmsDeep learningArtificial intelligenceMachine learningLearning algorithmImage processingImage reconstructionExisting methodsSegmentationPhase contrast imagesLearningContrast imagesImagesIntelligenceAlgorithmEmbeddingBrightfield microscopyTechniqueTransmission modalityProcessingApplications
2020
Label‐free histomorphometry of peripheral nerve by stimulated Raman spectroscopy
Hernández I, Yang W, Mohan S, Jowett N. Label‐free histomorphometry of peripheral nerve by stimulated Raman spectroscopy. Muscle & Nerve 2020, 62: 137-142. PMID: 32304246, DOI: 10.1002/mus.26895.Peer-Reviewed Original Research
2019
Stain-Free Resolution of Unmyelinated Axons in Transgenic Mice Using Fluorescence Microscopy
Mohan S, Hernández I, Selig M, Shibata S, Jowett N. Stain-Free Resolution of Unmyelinated Axons in Transgenic Mice Using Fluorescence Microscopy. Journal Of Neuropathology & Experimental Neurology 2019, 78: 1178-1180. PMID: 31642916, PMCID: PMC7967843, DOI: 10.1093/jnen/nlz099.Peer-Reviewed Original Research
2018
Fluorescent Reporter Mice for Nerve Guidance Conduit Assessment: A High‐Throughput in vivo Model
Mohan S, Hernández I, Wang W, Yin K, Sundback C, Wegst U, Jowett N. Fluorescent Reporter Mice for Nerve Guidance Conduit Assessment: A High‐Throughput in vivo Model. The Laryngoscope 2018, 128: e386-e392. PMID: 30098047, DOI: 10.1002/lary.27439.Peer-Reviewed Original ResearchConceptsHigh-throughput platformConduit performanceTissue processing stepsRobust axonal sproutingSciatic nerve transectionHigh throughputCryosection techniqueFluorescent reporter miceVivo mammalian modelsInterposition repairAxonal sproutingNerve transectionIsoflurane anesthesiaPeripheral neuronsAxonal ingrowthProcedure timeReporter miceNerve regenerationTransgenic miceVivo modelMiceMicroscopyVivo platformFluorescent proteinFluorescent microscopy