2022
Super-resolved fluorescence imaging of peripheral nerve
Hernández I, Mohan S, Minderler S, Jowett N. Super-resolved fluorescence imaging of peripheral nerve. Scientific Reports 2022, 12: 12450. PMID: 35864187, PMCID: PMC9304405, DOI: 10.1038/s41598-022-16769-0.Peer-Reviewed Original ResearchConceptsSuper-resolution microscopySuper-resolution imagingNanoscale resolutionSuper-resolution imaging techniquesEmission depletion (STED) microscopyNanoscale cellular structuresStructured illumination microscopyElectron microscopyVisible lightDiffraction limited resolutionDepletion microscopyMicroscopyFluorescence imagingNon-toxic meansIllumination microscopyBiomedical sciencesFluorescent dyeBrightfield microscopyFluorescent objectsSample preparationFluctuation microscopyAutomated stain-free histomorphometry of peripheral nerve by contrast-enhancing techniques and artificial intelligence
Coto Hernández I, Mohan S, Jowett N. Automated stain-free histomorphometry of peripheral nerve by contrast-enhancing techniques and artificial intelligence. Journal Of Neuroscience Methods 2022, 375: 109598. PMID: 35436515, DOI: 10.1016/j.jneumeth.2022.109598.Peer-Reviewed Original ResearchConceptsDeep learning algorithmsDeep learningArtificial intelligenceMachine learningLearning algorithmImage processingImage reconstructionExisting methodsSegmentationPhase contrast imagesLearningContrast imagesImagesIntelligenceAlgorithmEmbeddingBrightfield microscopyTechniqueTransmission modalityProcessingApplications
2019
Stain-Free Resolution of Unmyelinated Axons in Transgenic Mice Using Fluorescence Microscopy
Mohan S, Hernández I, Selig M, Shibata S, Jowett N. Stain-Free Resolution of Unmyelinated Axons in Transgenic Mice Using Fluorescence Microscopy. Journal Of Neuropathology & Experimental Neurology 2019, 78: 1178-1180. PMID: 31642916, PMCID: PMC7967843, DOI: 10.1093/jnen/nlz099.Peer-Reviewed Original Research
2018
Fluorescent Reporter Mice for Nerve Guidance Conduit Assessment: A High‐Throughput in vivo Model
Mohan S, Hernández I, Wang W, Yin K, Sundback C, Wegst U, Jowett N. Fluorescent Reporter Mice for Nerve Guidance Conduit Assessment: A High‐Throughput in vivo Model. The Laryngoscope 2018, 128: e386-e392. PMID: 30098047, DOI: 10.1002/lary.27439.Peer-Reviewed Original ResearchConceptsHigh-throughput platformConduit performanceTissue processing stepsRobust axonal sproutingSciatic nerve transectionHigh throughputCryosection techniqueFluorescent reporter miceVivo mammalian modelsInterposition repairAxonal sproutingNerve transectionIsoflurane anesthesiaPeripheral neuronsAxonal ingrowthProcedure timeReporter miceNerve regenerationTransgenic miceVivo modelMiceMicroscopyVivo platformFluorescent proteinFluorescent microscopy