2023
Structure Determination of SH2–Phosphopeptide Complexes by X-Ray Crystallography: The Example of p120RasGAP
Stiegler A, Boggon T. Structure Determination of SH2–Phosphopeptide Complexes by X-Ray Crystallography: The Example of p120RasGAP. Methods In Molecular Biology 2023, 2705: 77-89. PMID: 37668970, PMCID: PMC11059313, DOI: 10.1007/978-1-0716-3393-9_5.Peer-Reviewed Original ResearchConceptsSrc homology 2SH2 domain bindsSH2 domainDomain bindsNew molecular-level insightsSH2 domain proteinsMolecular-level insightsX-ray crystallographyX-ray diffraction studiesDomain proteinsPartner proteinsHomology 2Three-dimensional structureMolecular detailsStructure determinationSuitable crystalsCanonical interactionsVapour-diffusion methodCareful structural analysisDrop vapor diffusion methodCrystallographic studiesCrystallography studiesSH2-phosphopeptide complexesDiffraction studiesP120RasGAP
2001
G-Protein Signaling Through Tubby Proteins
Santagata S, Boggon T, Baird C, Gomez C, Zhao J, Shan W, Myszka D, Shapiro L. G-Protein Signaling Through Tubby Proteins. Science 2001, 292: 2041-2050. PMID: 11375483, DOI: 10.1126/science.1061233.Peer-Reviewed Original ResearchMeSH KeywordsActive Transport, Cell NucleusAdaptor Proteins, Signal TransducingAmino Acid SequenceAnimalsCell MembraneCell NucleusCells, CulturedCrystallography, X-RayGene Expression RegulationGTP-Binding Protein alpha Subunits, Gq-G11Heterotrimeric GTP-Binding ProteinsHumansIntercellular Signaling Peptides and ProteinsIntracellular Signaling Peptides and ProteinsIsoenzymesMembrane LipidsMiceModels, BiologicalMolecular Sequence DataNuclear Localization SignalsObesityPhosphatidylinositol 4,5-DiphosphatePhosphatidylinositol PhosphatesPhospholipase C betaPhosphorylationProtein Structure, TertiaryProteinsReceptor, Serotonin, 5-HT2CReceptors, MuscarinicReceptors, SerotoninRecombinant Fusion ProteinsSignal TransductionTranscription FactorsType C PhospholipasesConceptsTubby-like protein 3G protein signalingTubby proteinTubby domainTranscription regulatorsPlasma membranePhospholipase C-betaReceptor-mediated activationHeterotrimeric GTPSignal transductionGene expressionMolecular mechanismsTubbyC betaCarboxyl terminalCell nucleiMaturity-onset obesityProteinX-ray crystallographyProtein 3SignalingRegulatorMembranePhosphatidylinositolTransduction